Key to the invisible world found. ICTER scientists decipher two-photon vision

Two-photon vision is a novel method with great potential for the future of ophthalmic diagnostics. Although it has many advantages, it requires improvement in key areas. ICTER scientists have taken a step forward by improving this technology and opening up new perspectives in ocular medicine.

Imagine that instead of viewing an image through a lens, you look through a kaleidoscope that focuses invisible light to obtain a new range of colors. The photon, this ephemeral messenger of light, usually appears alone, but here it appears in a duet, which is the basis of two-photon vision. This extraordinary phenomenon in which the human eye, instead of perceiving traditional light, receives pulses of infrared lasers, the gateway to the invisible world.

However, the key to them is measuring the brightness of two-photon stimuli, which until now was only possible for visible light. ICTER scientists have made a breakthrough and determined the luminance value for infrared using photometric units (cd/m2). Thanks to this approach, it was possible to link the luminance of two-photon stimuli to a new physical quantity related to perceived brightness: the two-photon retinal illumination.

Research conducted by scientists from the International Centre for Eye Research (ICTER) with the participation of PhD student Oliwia Kaczkoś, PhD Eng. Katarzyna Komar and Prof. Maciej Wojtkowski have shown that the luminance of a two-photon stimulus can reach almost 670 cd/m2 in the safe range of laser power for the eye. The result is the paper entitled "Method for the determination of the luminance of two-photon vision stimuli," published in the journal Biomedical Optics Express.

Seeing the Invisible World

The human eye can receive stimuli from the surrounding world in the form of electromagnetic waves in the range of about 380 nm to 780 nm (from violet to red). Waves outside this range, such as infrared (above 780 nm) and ultraviolet (below 380 nm), are invisible to us without special devices, although they can affect the senses in other ways.

Every vision process follows the same path when a photon of visible light is absorbed by the visual pigment of the photoreceptor in the retina (the light-sensitive part of the eye). This event initiates a series of chemical reactions, as a result of which a quantum of light is converted into an electrical signal, processed in the brain.

Two-photon vision is a phenomenon in which the human eye can perceive ultrashort pulses of infrared lasers with a wavelength in the range of 800-1300 nm by absorbing two photons. This process causes isomerization of visual pigments, which leads to the perception of light with a wavelength corresponding to half the infrared wavelength. Although these lasers are outside the visible range of the spectrum, their effect on visual pigments allows infrared light to be recorded as different colors.

Two-photon vision differs from single-photon vision primarily in the way light is absorbed. In single-photon vision, each photon with a specific energy is absorbed by molecules in the eye, which allows light to be perceived in the visible range. In two-photon vision, on the other hand, two photons with half the energy are simultaneously absorbed by visual pigments, which leads to the perception of light with half the wavelength, which theoretically should not be visible.

Furthermore, the brightness of the two-photon stimulus varies with the square of the power of optical radiation, so light scattered in the eye will not be perceived. Brightness also depends on the focus of the beam on the observer's retina - the received stimuli are sharper and have better contrast than in the case of "normal," single-photon vision.

ICTER scientists have long been studying the phenomenon of two-photon vision, were the first in the world to describe it, and now they have made another groundbreaking discovery.

A novel method for determining the brightness of two-photon stimuli

Two-photon vision shows potential in two key areas: medical diagnostics and virtual/augmented reality (VR/AR). It can be used for advanced diagnostic tests, especially in neurology and ophthalmology, where infrared pulses allow for safe monitoring of visual functions without the need to use visible light. On the other hand, this phenomenon allows the creation of new, realistic visual experiences by manipulating light stimuli from the infrared range, opening up new possibilities in interaction with virtual images (VR/AR).

All future applications of this phenomenon require knowledge of the luminance of two-photon stimuli, but the luminous efficiency function V(λ) outside the visible range is unknown. A non-standard approach to quantifying the luminance of two-photon stimuli is necessary, e.g., using infrared - which is what ICTER scientists did.

The method presented in the paper allowed the expression of the brightness of two-photon stimuli in photometric units. Thanks to the measurements performed, the scientists were able to demonstrate the relationship between the power of the infrared beam and the power of the visible beam, which was subjectively adjusted so that both were perceived as having the same luminance. Using the relationship between the power density of the VIS laser and the luminance of the projected stimuli, it was possible to determine the subjective luminance of the infrared stimuli using photometric units (cd/m2). These results emphasize the nonlinear nature of two-photon vision, which is in agreement with previous studies.

The study aimed to develop a repeatable method for determining the brightness of stimuli for two-photon vision. Standard methods do not allow this to be done outside the visible spectrum of light, but our research opens the door to achieving this goal, which is necessary for further research and development of applications of this phenomenon in medical diagnostics and augmented reality (AR) and virtual reality (VR) technologies. The new approach will also enable comparison of the brightness of two-photon stimuli with traditional displays based on standard, single-photon vision - says Oliwia Kaczkoś, ICTER PhD student and optometrist, lead author of the study.

A platform for further discoveries

The result of the research is the proposal of a completely new physical quantity, called two-photon retinal illumination, which is sufficient to describe systems emitting two-photon stimuli. This relationship allowed the prediction of the luminance values ​​of two-photon stimuli, which could reach 670 cd/m2 in the safe laser power range of the human eye without adaptive optics (AO) correction.

Moreover, ICTER scientists documented twice the repeatability for measurements made on a background with a luminance of 10 cd/m2. This is crucial for the development of future technologies, such as two-photon retinal displays, which could be used in augmented reality (AR) glasses or in advanced diagnostic tools such as two-photon microperimetry.

Authors of the paper "Method for determination of luminance of two-photon vision stimuli": Oliwia Kaczkoś, Agnieszka Zielińska, Jacek Pniewski, Maciej Wojtkowski, and Katarzyna Komar.

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ICTER, or the International Centre for Translational Eye Research, is a research, development and innovation centre at the Institute of Physical Chemistry of the Polish Academy of Sciences (IChF), located in Warsaw, Poland. ICTER was established in 2019 to develop cutting-edge technologies to support the diagnosis and therapy of eye diseases, based on funding from the International Research Agendas Programme of the Foundation for Polish Science, co-financed by the European Union - European Regional Development Fund. The centre is currently implementing the MAB FENG grant of the Foundation for Polish Science. In 2024, IChF has won a prestigious grant under the Teaming for Excellence / WIDERA program of Horizon Europe, which will enable ICTER unit to develop into a European centre of excellence. The centre’s website is: www.icter.pl.