Job details Print page

A fundamental problem in tissue engineering is the fabrication of tissues with an embedded vasculature—functional at all relevant length scales, down to the cellular scale where the smallest vessels are to deliver nutrients and remove metabolic byproducts. Current approaches to engineering of capillary networks typically rely on spontaneous selfassembly of the endothelial cells (EC), suspended in an external hydrogel–mimicking the extracellular matrix of the native tissue–into branched tubular networks. However, such self-assembled networks are typically far from optimal. In particular, (i) they develop slowly in time (the cells need to migrate over relatively large distances), (ii) they are heterogeneous and weakly percolated, (iii) they do not allow any external control over the vessel distribution, connectivity and general network topology. In this project, we will develop a new method of vascular tissue engineering based on the use of EC ‘seeds’, that is aggregates of ECs or microbeads coated with ECs, suspended in an external 3D hydrogel matrix. It is known that EC aggregates tend to spontaneously ‘sprout’ into the matrix, i.e., develop tubular protrusions, precursors of the microvessels. In the project, we will use the sprouting seeds as building blocks of larger, interconnected vascular constructs. To this end, we will develop a new technique of directed-assembly of EC-coated microbeads into ordered arrays using magnetic forces. We will assemble the arrays inside a hydrogel-filled microfluidic chamber allowing longterm culture and facilitating confocal time-lapse imaging of the developing microvasculature. To rationalize the observed topologies, we will develop a data-driven theoretical model of the dynamically evolving vascular networks. In particular, based on the theory cross-validated with experimental data, we will determine the optimal conditions for the development of networks of highest possible connectivity and viability. Finally, we will use our EC bead-based experimental platform to study the development of microvasculature in cancer tissues. For this purpose we will co-culture the endothelial seeds with cancer cells and characterize the impact of the outgrowing microtumors on the topology of the ingrowing capillary networks. The platform enables tracking of individual cancer cells upon their extravasation into co-cultured micro-vessels, which we will use to quantify the metastasic potential of cancer cells. We will use various sub-types of breast cancer cells and study the response of the ensuing microtumors to anti-cancer drugs as a proof-of-concept of a personalized approach to cancer treatment. Altogether, in the project, we will establish a highly controlled and versatile tissue-on-chip platform for studying basic mechanisms of angiogenesis in healthy and diseased tissues as well as for future applications in personalized cancer therapies.

Post-doc will be will be responsible for performing the cell culture experiments and developing a variety of experimental tools, including optimization of confocal imaging of sprouting vessels, designing microfluidic devices, developing megnetic manipulation method, performing microfluidic experiments. Post-doc will closely collaborate with the theory team regarding data post-processing
It is expected that the successful candidate will be able to perform experiments independently, plan the workflow and participate in the decision-making process.
Responsibilities:
• Designing, planning, and carrying out experimental work independently, maintaining regular research notes.
• Preparation of manuscripts and reports.
• Scientific initiative and contribution through regular reporting and publishing, as well as presenting at group meetings as well as in national and international conferences;

• Providing help and supervision to junior members of the group;
• Contribution to the efficient functioning of the lab, including necessary organizational tasks.

We offer a post-doctoral position (full-time employment) in the Institute of Physical Chemistry PAS with a gross salary of around 9600 PLN gross per month financed from the NCN OPUS 23 Project No. 2022/45/B/ST8/03675 100% of the work time of Postdoc will be devoted to the realization of the proposed project. The offer is for 12 months with possible extensions based on the performance (the maximum duration of the contract is planned for 36 months).

The successful candidate will benefit in the form of new knowledge and methods added to his/her toolbox. The project deals with biology, tissue engineering, physics, material sciences , so a broad range of candidates will find new knowledge to be gained upon the realization of the project.

We plan to publish the results of the work in high-impact factor journals.
The successful candidate will be able to take advantage of all programs in the IPC PAS, including Young Scientists of IPC PAS competition and Mobility of Young Scientists of IPC PAS competition.
The institute provides an opportunity to participate in the ERASMUS+ programme.

1. PhD degree in biological, chemical or physical sciences, obtained in the year of employment in the project or within 7 years before 1 January of the year of employment in the project. For researchers with multiple PhDs, the date of the first PhD award is deemed the reference date. Extension might apply according to National Science Centre, Poland, rules.
2. Interdisciplinary experience in microfluidics and organ-on-chip technology. Advanced knowledge in molecular biology is a necessary prerequisite. Knowledge of general techniques used in tissue engineering (e.g., 3D cell culture) would be desired. General background in engineering or bioengineering would be advantageous.
3. Publication record and rich scientific experience.
4. Ability to work independently as well as in a group
5. Ability to communicate fluently in English (written and spoken).
6. Strong motivation to work and pursue scientific questions, creativity, and analytical thinking.

The Commission will take into account the following criteria:
a) competencies of candidates for specific tasks in a research project,
b) previous scientific achievements of candidates,
c) awards and distinctions of the candidate resulting from the conducted research.
The commission evaluates applications on a point scale.